Comparative Evaluation of the Application of Polystyrene Microspheres and Polystyrene Carboxyl Microspheres in Biotechnology – Concentrating On Nucleic Acid Removal.

(LNJNbio Polystyrene Microspheres)

In the field of modern biotechnology, microsphere products are widely utilized in the removal and purification of DNA and RNA as a result of their high certain area, good chemical security and functionalized surface properties. Amongst them, polystyrene (PS) microspheres and their derived polystyrene carboxyl (CPS) microspheres are among the two most commonly examined and used products. This post is supplied with technological assistance and data analysis by Shanghai Lingjun Biotechnology Co., Ltd., aiming to systematically contrast the performance distinctions of these two types of materials in the process of nucleic acid extraction, covering key signs such as their physicochemical buildings, surface area modification ability, binding effectiveness and healing rate, and highlight their appropriate circumstances through speculative data.

Polystyrene microspheres are homogeneous polymer particles polymerized from styrene monomers with excellent thermal stability and mechanical stamina. Its surface area is a non-polar framework and typically does not have energetic functional teams. Consequently, when it is directly used for nucleic acid binding, it needs to rely on electrostatic adsorption or hydrophobic activity for molecular addiction. Polystyrene carboxyl microspheres introduce carboxyl useful groups (– COOH) on the basis of PS microspheres, making their surface area efficient in additional chemical combining. These carboxyl groups can be covalently bound to nucleic acid probes, proteins or various other ligands with amino groups through activation systems such as EDC/NHS, therefore achieving more stable molecular fixation. Consequently, from an architectural point of view, CPS microspheres have much more benefits in functionalization potential.

Nucleic acid extraction normally consists of steps such as cell lysis, nucleic acid release, nucleic acid binding to solid stage service providers, cleaning to eliminate impurities and eluting target nucleic acids. In this system, microspheres play a core role as strong stage carriers. PS microspheres generally rely upon electrostatic adsorption and hydrogen bonding to bind nucleic acids, and their binding performance is about 60 ~ 70%, yet the elution effectiveness is reduced, only 40 ~ 50%. In contrast, CPS microspheres can not just make use of electrostatic results but also accomplish even more solid fixation via covalent bonding, decreasing the loss of nucleic acids throughout the cleaning process. Its binding performance can reach 85 ~ 95%, and the elution efficiency is also raised to 70 ~ 80%. Furthermore, CPS microspheres are likewise dramatically better than PS microspheres in regards to anti-interference capability and reusability.

In order to validate the performance differences between both microspheres in real operation, Shanghai Lingjun Biotechnology Co., Ltd. carried out RNA extraction experiments. The speculative examples were stemmed from HEK293 cells. After pretreatment with conventional Tris-HCl buffer and proteinase K, 5 mg/mL PS and CPS microspheres were made use of for removal. The results revealed that the average RNA return drawn out by PS microspheres was 85 ng/ μL, the A260/A280 ratio was 1.82, and the RIN worth was 7.2, while the RNA yield of CPS microspheres was boosted to 132 ng/ μL, the A260/A280 proportion was close to the ideal worth of 1.91, and the RIN worth got to 8.1. Although the operation time of CPS microspheres is somewhat longer (28 mins vs. 25 minutes) and the price is greater (28 yuan vs. 18 yuan/time), its extraction high quality is substantially improved, and it is better for high-sensitivity detection, such as qPCR and RNA-seq.

( SEM of LNJNbio Polystyrene Microspheres)

From the viewpoint of application scenarios, PS microspheres are suitable for large screening tasks and preliminary enrichment with reduced needs for binding specificity because of their affordable and easy procedure. However, their nucleic acid binding capability is weak and easily affected by salt ion focus, making them inappropriate for lasting storage or duplicated usage. On the other hand, CPS microspheres are suitable for trace example extraction due to their rich surface practical groups, which assist in additional functionalization and can be utilized to construct magnetic bead detection sets and automated nucleic acid extraction platforms. Although its prep work procedure is relatively intricate and the price is relatively high, it shows more powerful adaptability in clinical research study and scientific applications with rigorous demands on nucleic acid extraction efficiency and purity.

With the quick development of molecular diagnosis, genetics editing, liquid biopsy and various other areas, higher requirements are positioned on the performance, pureness and automation of nucleic acid removal. Polystyrene carboxyl microspheres are slowly changing conventional PS microspheres because of their outstanding binding efficiency and functionalizable qualities, ending up being the core option of a new generation of nucleic acid removal materials. Shanghai Lingjun Biotechnology Co., Ltd. is likewise continuously enhancing the particle size circulation, surface area thickness and functionalization effectiveness of CPS microspheres and developing matching magnetic composite microsphere products to fulfill the demands of professional diagnosis, scientific research study establishments and commercial customers for premium nucleic acid extraction services.

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Our products are widely used in many fields, such as medical testing, genetic testing, university research, genetic breeding and more. We not only provide products but can also undertake OEM, ODM, and other needs. If you need extraction of rna, please feel free to contact us at sales01@lingjunbio.com.

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